Acute Toxicity

  • Independent testing
    Envirolyte systems and generated solutions (anolyte and catolyte) have been put through extensive series of independent tests and on-site trials including hospitals, breweries, water systems and agricultural environments.

  • Safer world
    Being environmentally friendly, completely safe, non-toxic and non-irritant Anolyte is welcome where traditional chemicals fail to produce the desired results or can't be applied at all.

  • Better alternative
    The conclusions prove that Anolyte being a low-cost and powerful disinfectant is set to become the preferred solution for many sterilisation, disinfecting and water purification procedures.


Undertaken by Insitute of Chemistry of Tallinn Technical University, Estonia.

Daphnia magna 24 hours acute toxity tests were carried out according to Finnish standard SFS 5062


The dilutions used were:

  • Anolyte medium : 1:100, 1:500 and 1:1000
  • Anolyte strong: 1:50, 1:200 and 1:500


The acute toxity of Anolyte

Anolyte typeDilutionEC50; % (confidence limit)EC50; % for initial anolyte
Anolyte medium1:10025 (22.5-28)0.25
 1:500Not toxic 
 1:1000Not toxic 
Anolyte strong1:5013 (11-16)0.26-0.28
 1:20056 (52-59) 
 1:500Not toxic 


The toxity test demonstrated that there were no acute effects for the anolyte diluted 1:500 or more. No significant differences in the toxity of two anolytes (strong and medium) were observed.